Determining the enterotoxin genes and methicillin resistance in Staphylococcus Aureus isolated from goat milk and its products

نویسندگان

چکیده

Milk and its products can be frequently contaminated with enterotoxigenic methicillin-resistant S. aureus, in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials (50 goat milk, 25 cream, cheese) were collected from 65 livestock farms Erzurum. All samples analyzed tested selectively according to EN ISO 6888-1 procedure standard. The obtained isolates examined PCR terms of nuc, Panton-Valentine Leukocidin (PVL), mecA, enterotoxin genes. aureus was detected 4 50 (8%) milk samples, 2 cream 3 (12%) cheese 9 total (9%). While incidence methicillin resistance found 3% by disc diffusion method, mecA gene 2% PCR. genes, 8% (4/50) 12% (3/25) (2/25) contained at least one gene. total, 9% aureus. conclusion, consumption positive aureus-containing raw dairy that have not been adequately heat-treated, or are kept proper storage conditions pose danger public health

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Enterotoxin gene profiles among Staphylococcus aureus isolated from raw milk

Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the nine Staphylococcal enterotoxins (SEs) and enterotoxin gene profiles in S. aureus isolates derived from raw bovine milk....

متن کامل

Detection of Classic Enterotoxin Genes and Coagulase Gene Typing of Staphylococcus Aureus Isolated from Raw Cow Milk in Isfahan

Background and Aims: Staphylococcus aureus (S. aureus) is considered as one of the most dangerous pathogenic bacteria due to the production of extracellular toxins. The objective of this study was to determine the prevalence of S. aureus and to characterize the recovered strains for their enterotoxin-producing genes in raw cow milk. Materials and Methods: During 9 months duration of the s...

متن کامل

Distribution Patterns of Methicillin Resistance Genes (mecA) in Staphylococcus aureus Isolated from Clinical Specimens

There is a growing concern about the application of molecular methods in epidemiological studies of infectious diseases. The objective of this study was to determine the genetic stability of methicillin resistance genes in Staphylococcus aureus for the evaluation of resistance strain distribution. One hundred and fifteen S. aureus isolates from patients with staphylococcal infection were collec...

متن کامل

The Frequency of Staphylococcus aureus Classical Enterotoxin Genes in Raw Milk Samples in Zanjan, Iran

Background: Staphylococcus aureus is one of the major causes of food poisoning. Since milk is a nutritious source of proteins and vitamins, it could provide the optimal conditions for the growth of several bacterial pathogens, such as S. aureus. The present study aimed to assess the frequency of Staphylococcus aureus classical enterotoxin genes in raw milk samples in Zanjan, Iran. Methods: In ...

متن کامل

PCR-mediated identification of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains isolated from the nasal cavity

Staphylococcus aureus is colonized in the human nasal cavity and would contaminate hospital and therapeutic environments. This bacterium has a genetic diversity in terms of resistance to antimicrobial agents. Therefore, the purpose of this study was identificatied of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains which has been isolated from the nasal cavity. 189 pa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: MANAS journal of engineering

سال: 2022

ISSN: ['1694-7398']

DOI: https://doi.org/10.51354/mjen.1185346